-  Run an ERC 
-  Run a DRC 
-  Check stop mask - do you want tenting?  
-  Sometimes its cheaper to have both sides masked the same so mirror any stop masks like 'heat sink' planes onto the other side 
-  Check the layout with Gerbv or another gerber viewer  - 
-  are the bottom and top lined up? 
-  Are the holes in the right place? 
-  silkscreen looking good, nothing overlapping? 
-  bottom/top components there? 
 
-  (optional) Sometimes its cheaper to have fewer drills, so edit the drill file to keep the tool number low (sometimes your CAD program can do this too) 
-  Do you need scoring? 
-  Do you need panelizing? If so, scored or tab-route? 
-  If you panelize, make sure to send your solderpaste layer for them to tile at the same time 
-  Do you need lead free? 
-  Did you stick some fiducials in there? 
-  What color mask and silk? For me, blue and white. 
-  What finish? I like Gold Immersion 
-  For panelization, 3mm holes in the corners will work best with an STP-350 printer